Table 2. Yields of different IgG and IgG-like formats of SH313-F9 variants produced in HEK293–6E cells.
| Antibody | Format | Initial format | Yield 10 mL scale [mg/L] |
Yield 50–100 mL scale [mg/L] |
|---|---|---|---|---|
| SH313-F9 | IgG | scFv | 2.7 | 8 |
| MiS200-B3 | IgG | scFv | 7.0 | |
| MiS200-G12 | IgG | scFv | 6.1 | |
| MiS200-D12 | IgG | scFv | 16.0 | |
| MiS201-C3 | IgG | scFabΔC | 10.3 | |
| MiS201-F2 | IgG | scFabΔC | 4.0 | |
| MiS179-F5 | IgG | scFabΔC | 8.7 | |
| MiS201-F6 | IgG | scFabΔC | 0.2 | |
| MiS203-C1 | IgG | FabΔC | 0.1 | 11 |
| MiS203-C9 | IgG | FabΔC | 0.1 | 7 |
| MiS196-D5 | IgG | FabΔC | 0.4 | 5 |
| SH313-F9 | scFv-Fc | scFv | 31.1 | 303 |
| MiS200-B3 | scFv-Fc | scFv | 20.4 | |
| MiS200-G12 | scFv-Fc | scFv | 19.3 | |
| MiS200-D12 | scFv-Fc | scFv | 27.4 | |
| SH313-F9 | scIgG | scFv | 9.8 | 106 |
| MiS201-C3 | scIgG | scFabΔC | 5.3 | |
| MiS201-F2 | scIgG | scFabΔC | 25.0 | |
| MiS179-F5 | scIgG | scFabΔC | 10.3 | |
| MiS201-F6 | scIgG | scFabΔC | 14.7 |