Table 3. Affinity determined by SPR.
| Antibody | kon [1/Ms] | koff [1/s] | KD [M] | χ2 | Affinity compared with SH313-F9 IgG |
|---|---|---|---|---|---|
| SH313-F9 IgG | 1.38 × 104 | 2.20 × 10−3 | 1.59 × 10−7 | 2.2 | 1.0 |
| SH313-F9 scFv-Fc | 7.18 × 103 | 3.30 × 10−3 | 4.18 × 10−7 | 5.3 | 0.4 |
| SH313-F9 scIgG | 7.18 × 103 | 3.28 × 10−3 | 4.56 × 10−7 | 8.7 | 0.3 |
| MiS203-C1 IgG | 3,03 × 104 | 6.11 × 10−4 | 2.02 × 10−8 | 0.3 | 7.9 |
| MiS203-C9 IgG | 3,96 × 104 | 5.22 × 10−4 | 1.32 × 10−8 | 1.2 | 12.0 |
| MiS196-D5 IgG | 3,75 × 104 | 5.42 × 10−4 | 1.45 × 10−8 | 1.0 | 11.0 |
kon, off-rate; koff, off-rate; KD dissociation equilibrium constant (affinity); χ2, chi square value (curve fitting)