Figure 6. XMetD is not an antagonist of IGF-1 dependent Akt phosphorylation. CHO-hIGF-1R cells were preincubated for 30 min at 37 °C with 333 nM of either XMetD or control IgG followed by a 10 min incubation with increasing concentrations of IGF-1. Phosphorylation of Akt was measured using an electrochemiluminescence based assay. Mean ± SD of triplicate determinations are shown.