Figure 8. XMetD inhibition of mINSR signaling. CHO-mINSR (B isoform) cells were preincubated for 30 min at 37 °C with 333 nM of either XMetD or control IgG followed by a 10 min incubation with increasing concentrations of insulin. Phosphorylation of Akt was measured using an electrochemiluminescence based assay. Mean ± SD of triplicate determinations are shown.