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. Author manuscript; available in PMC: 2014 Feb 20.
Published in final edited form as: Fertil Steril. 2013 May;99(6):1523–1533. doi: 10.1016/j.fertnstert.2013.03.043

Figure 2. Two-Step Culture System To Obtain Growing Human Follicles.

Figure 2

A: An outline of the preparation of human ovarian cortical biopsies to initiate development of primordial follicles (Step 1) and after 6 days in serum free medium dissect out multi laminar growing follicles and grow these individually (16).

Photomicrograph of freshly prepared micro-cortex before culture (B) and after 6 days of culture (C) followed by dissection to isolate multilaminar follicles with theca cells attached (D). Isolated follicles can be cultured individually as outlined in A and antral formation occurs during a further 4 day culture period.