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. Author manuscript; available in PMC: 2014 Feb 20.
Published in final edited form as: Fertil Steril. 2013 May;99(6):1523–1533. doi: 10.1016/j.fertnstert.2013.03.043

Figure 3.

Figure 3

Left panel depicts representative histological images of isolated rhesus macaque primordial follicles after encapsulation in alignate at Day 0 (A and B), cultured for 6 days without encapsulation (C and D), or cultured for 6 days while encapsulated in 0.5% (E and F) or 2% (G and H) alginate. Scale bar = 50 μm; asterisks denote oocytes. From reference (42) Right panel represents morphology and histology of isolated human primordial follicles encapsulated in alginate before in vitro culture (A), after 7 days in vitro (B), a cryopreserved follicle after 7 days in vitro (C), and a follicle isolated from cryopreserved-thawed ovarian cortex after 7 days in culture (D). Images are at 40X magnification. Semi-thin sections of a cryopreserved isolated follicle (E, 1000X) and a follicle isolated from frozen-thawed ovarian cortex (F, 400X) after 7 days of culture encapsulated in alginate. From reference (53).