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. 2014 Feb 19;9(2):e87614. doi: 10.1371/journal.pone.0087614

Figure 5. Secretory miRNA in colon culture medium.

Figure 5

miRNA 23b in conditioned medium was measured by miRNA plate assay chemiluminescence method (Fig. 5A) and qRT-PCR (Fig. 5B). Cy3 labelled miRNA 23b uptake by macrophage (Fig. 5C). miRNA 23b uptake at 4°C (Fig. 5D), miRNA 23b uptake at pH 7.0 (Fig. 5E) and pH 6.5 (Fig. 5F). F-actin (FITC - green), nuclear staining (DAPI - blue) and Cy3 labelled miRNA 23b (TRITC -filter setting used) are stained and separate pictures were taken at 60× for each filter and merged respectively. Values represent means ± SEM of n = 6/group. *p<0.05, **p<0.001 and ***p<0.0001. Scale bar = 10 µm.