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System suitability parameters.
| Parameter | Compound | |
|---|---|---|
| Emtricitabine | IS | |
| Retention time (Rt) | 4.39 | 6.58 |
| Tailing factor (T) | 1.01 | 1.13 |
| †Injection repeatability (RSD) | 0.752 | 0.623 |
| ‡Resolution (R s) | — | 7.32 |
| Capacity factor (K′) | 5.92 | 6.30 |
| Theoretical plates (N) | 4976 | 5318 |
| Asymmetry | 1.23 | 1.36 |
†RSD of peak areas of six consecutive injections at a concentration of 2.0 and 0.2 µg/mL of emtricitabine and IS, respectively.
‡Resolution between emtricitabine and IS.
