Figure 1. Predicting glycan motifs on proteins using multiple lectin measurements.

We begin with an array of antibodies immobilized on a solid support. We then incubate a biological solution on the antibody array, and the antibodies capture their target glycoproteins. In parallel experiments, we use a series of lectins to probe the glycans on the captured proteins. The motif prediction algorithm uses these measurements and information from a database of lectin-glycan interactions to calculate the likelihood of the presence of various glycan motifs.