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Immunoblotting of AQP5 from transfected cells and tissue homogenates. (a) A band at approximately 24 kDa was common to all samples (arrowhead) except for the untransfected control. (b) When the AQP5 antibody was preabsorbed with the antigen peptide prior to immunoblotting, the band at approximately 24 kDa largely disappears. TF, AQP5-transfected COS7 cells; control, untransfected COS7 cells.
