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Semi-quantitative immunoblotting of AQP5 in pilocarpine- or IPR-administered parotid glands. (a) Ten micrograms of tissue homogenate protein was prepared from parotid glands and electrophoresed. AQP5-specific bands (arrowhead) on the blot were quantified. (b) Band intensities are expressed as arbitrary units. Data are expressed as the mean±SEM (n=3). Statistical analysis was performed using the Student’s t-test.
