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Semi-quantitative immunoblotting of AQP5 in pilocarpine- or IPR-administered submandibular glands. (a) Ten micrograms of tissue homogenate protein was prepared from submandibular glands and electrophoresed. AQP5-specific bands (arrowhead) on the blot were then quantified. (b) Band intensities are expressed as arbitrary units and data are the mean±SEM (n=3). Statistical analysis was performed using the Student’s t-test.
