Table 3. Calibration and mass spectra interpretation for diazepam, silylated temazepam and flunitrazepam standards.

Drug	Regressionequation	R2 value	LODa/ng	LOQb/ng	Majorions/m/z	Interpretation	% abundance
Diazepam	Y = 27.7× +0.27	0.978	0.571	0.643	256	[M-CO]+ or[M-(H+HCN)]+	100
					283	[M-H]+	97
					221	[M-(CO+Cl)]+ or[M-(H+HCN+Cl)]+	33
Flunitrazepam	Y = 12.02×–0.797	0.983	0.714	1.286	312	[M-H]+	100
					286	[M-HCN]+	97
					266	[M-(H+NO2)]+ or[M-(CO+F)]+ or[M-(H+HCN+F)]+	61
Silylatedtemazepam	Y = 35.7× +0.064	0.977	0.500	0.714	343	[M-(H+CO)]+ or[M-(2H+HCN)]+	100
					283	[M-(OSi(CH3)3+H)]+	31
					257	[M-NCHOSi(CH3)3]+	36

^aLimit of detection on column, IUPAC method.

^bLimit of quantification on column, IUPAC method.