Table 4. Method validation and Kruskall-Wallis U test results from spiked beverages.

Spiked beverages		a , bLOD/ng	b , cLOQ/ng	dRecovery % ± eSD	fIs the difference significant at 95% CI?
					RT storage	4°C storage
Diazepam	Bacardi Breezer	0.25	0.714	89±7.9	No	No
	Beer	0.571	1.43	84±8.4	No	No
	Vodka	0.286	1	99±2.9	No	No
	White wine	0.071	0.156	86±5.4	No	No
	J2O	0.143	0.5	86±6.5	Yes	No
Flunitrazepam	Bacardi Breezer	0.528	1.71	77±15	No	No
	Beer	1.07	1.5	101±20	No	Yes
	Vodka	0.356	1.14	97±4.1	No	Yes
	White wine	0.082	0.273	56±5.3	Yes	Yes
	J2O	0.643	2.11	85±6.5	Yes	Yes
Silylated temazepam	Bacardi Breezer	0.143	0.431	84±2.5	Yes	Yes
	Beer	0.071	0.357	97±13	Yes	No
	Vodka	0.143	0.357	90±6.7	Yes	Yes
	White wine	0.071	0.143	81±4.1	Yes	Yes
	J2O	0.214	0.693	105±10	Yes	Yes

^aLimit of detection,

^bIUPAC method,

^climit of quantification,

^dn = 3,

^estandard deviation,

^fKruskall-Wallis U test at 95% confidence interval.