Skip to main content
. 2014 Feb 19;9(2):e89148. doi: 10.1371/journal.pone.0089148

Figure 1. Ang-1 inhibited LPS-induced cytokines production in P815 mast cells.

Figure 1

Quantitative RT-PCR (A and B) and ELISA (C and D) were employed for detection of mRNA and the secretion of cytokines production in duplicates, respectively. A and C: LPS dramatically increased TNF-α mRNA production and protein secretion in P815 mast cells (column 2 versus column 1). Addition of Ang-1 abrogated the induction of LPS on mast cells (column 3 versus column 2). Soluble form of Tie2 (sTie-2) and RGD reversed the inhibition of Ang-1 on LPS-induced TNF-α production (column 4, 5 versus column 3). *P<0.05. B and D: IL-6 mRNA and secretion were both significantly increased in mast cells upon LPS treatment. Ang-1 could decrease the induction while sTie-2 and RGD exerts opposite effects. *P<0.05.