Figure 3. Relative expression levels of PyIGFBP in embryos/larvae (A) and adult tissues (B) of the Yesso scallop.

Three biological replicates were performed for each developmental stage (n>500 for every replicate), and 12 for each adult tissue. Three technical replicates were conducted for each PCR. The comparison of the expression levels of PyIGFBP among different developmental stages and among adult tissues was performed using one way ANOVA with a post-hoc test. Bars with different superscripts indicate significant differences (P<0.05).