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(A)Western blot was performed to determine HMGB1 protein expression in HCCLM6, HCCLM6/mock, HCCLM6/S1, HCCLM6/S2, HCCLM3,HCCLM3/mock,HCCLM3/HMGB1+); Tubulin was used as a control. (B)qRT-PCR was performed to determined HMGB1 mRNA expresion in HCCLM6, HCCLM6/mock, HCCLM6/S2, HCCLM3, HCCLM3/mock, HCCLM3/HMGB1+).
