247 |
Primary human ovarian cancer cells isolated from ascites |
RPMI-1640 + 10% FBS |
3T3 |
Murine embryonic fibroblasts |
DMEM + 10% FBS |
448 |
Primary human ovarian cancer cells isolated from ascites |
RPMI 1640 + 10% FBS |
4T1 |
Murine breast carcinoma |
DMEM + 10% FBS |
5637 |
Human bladder carcinoma |
RPMI 1640 + 10% FBS |
5Y |
Human neuroblastoma |
MEM:F12 + 2 mM l-glutamine + NEAA + 10% FBS |
67NR, 168FARN, 4T07, 66cl4 |
Murine breast cancer progression series. 67NR forms primary tumors; 168FARN forms micrometastases in lymph nodes and 4T07 micrometastases to blood, lymph nodes and lungs. 66cl4 and 4T1 are fully metastatic and forms visible lung nodules (14, 15) |
DMEM + 10% FBS |
BE2 |
Human neuroblastoma |
MEM:F12 + 2 mM l-glutamine + NEAA + 10% FBS |
Cos-7 |
African green monkey kidney fibroblast-like |
DMEM + 10% FBS |
DOV13 |
Human ovarian cancer |
RPMI-1640 + 10% FBS |
HaCaT |
Human nontransformed keratinocyte |
DMEM + 10% FBS |
Hek293 |
Human nontransformed embryonic kidney |
DMEM + 10% FBS |
Hep3B |
Human hepatocellular carcinoma |
MEM + 10% FBS |
HepG2 |
Human hepatocellular carcinoma |
MEM + 10% FBS |
HEY |
Human ovarian cancer |
RPMI-1640 + 10% FBS |
HMEC |
Nontransformed human mammary epithelial |
DMEM + 10 μg/ml insulin + 10% FBS |
HMEC-1 |
Human dermal microvascular endothelial |
MCDB-131 + 10% FBS + 1 μg/ml hydrocortisone + 10 ng/ml EGF + 2 mM l-glutamine |
HMEpC |
Primary human mammary epithelial cells (purchased from Cell Applications, Inc.) |
Mammary epithelial growth medium (Cell Applications, Inc.) |
HPrEpC |
Primary human prostate epithelial cells (purchased from Cell Applications, Inc.) |
Epi growth medium (Cell Applications, Inc.) |
HT-29 |
Human colorectal adenocarcinoma |
McCoy's 5A + 10% FBS |
IGROV |
Human ovarian cancer |
RPMI-1640 + 10% FBS |
L6 |
Rat myoblast |
DMEM + 10% FBS |
Mammary epithelial cells (TβRII WT and KO) |
Nontransformed murine mammary epithelial |
DMEM:F12 + 2.5 mM l-glutamine + 10% adult bovine serum (ABS) |
Mammary fibroblast cells (TβRII WT and KO) |
Nontransformed murine mammary fibroblast |
DMEM:F12 + 2.5 mM l-glutamine + 10% ABS |
MCF10A |
Nontransformed human mammary epithelial |
F12:DMEM + 10 μg/ml insulin + 0.5 μg/ml hydrocortisol + 20 ng/ml EGF + 100 ng/ml cholera toxin + 5% horse serum |
MCF10A T1k, Ca1h, Ca1a |
Human breast cancer progression series. MCF10A is nontumorigenic, T1k forms ducts that can develop hyperplastic lesions; Ca1h forms low-grade, well-differentiated carcinomas; and Ca1a forms poorly differentiated, metastatic carcinomas (16). |
DMEM:F12 + 10 μg/ml insulin + 0.5 μg/ml hydrocortisol + 20 ng/ml EGF + 100 ng/ml cholera toxin + 5% horse serum |
MCF7 |
Human breast adenocarcinoma |
MEM + 0.01 mg/ml insulin + 10% FBS |
MDA-MB-231 |
Human breast cancer |
MEM + sodium pyruvate + nonessential amino acids (NEAA) + 10% FBS |
MDCK |
Nontransformed canine kidney epithelial |
MEM + 10% FBS |
MEEC |
Murine embryonic endothelial |
MCDB-131 + 15% FBS + 2 mM l-glutamine + 1 mM sodium pyruvate + 100 μg/ml heparin + 15 μg/ml endothelial cell growth supplement |
Mv1Lu, DR, R1b |
Nontransformed mink lung epithelial Mv1Lu is wild-type, DR lacks TβRII and ALK5, and R1b lacks ALK5 (17) |
DMEM + 10% FBS |
NMuMG |
Murine nontransformed mammary epithelial |
DMEM + 4.5g/l-glucose + 10 mg/ml insulin + 10% FBS |
NOSE7 |
Normal ovarian surface epithelial |
RPMI-1640 + 10% FBS |
Ovca 429 |
Human ovarian cancer |
RPMI + 10% FBS |
Panc-1 |
Human pancreatic cancer |
DMEM + 10% FBS |
PASMC |
Murine pulmonary artery smooth muscle cells |
RPMI-1640 + 10% FBS |
PC3 |
Human prostate adenocarcinoma |
F12 + 10% FBS |
RT4 |
Human transitional cell papilloma |
McCoy's 5A + 10% FBS |
SHEP |
Human neuroblastoma |
DMEM + 2 mM l-glutamine + NEAA + 10% FBS |
SJD.1 |
Adult zebrafish caudal fin fibroblast |
High glucose DMEM + 15% heat inactivated FBS, 28 °C |
SKNAS |
Human neuroblastoma |
DMEM + 2 mM l-glutamine + NEAA + 10% FBS |
SW480 |
Human colorectal adenocarcinoma |
DMEM + 10% FBS |
SW620 |
Human colorectal adenocarcinoma |
DMEM + 10% FBS |
T24 |
Human transitional cell carcinoma |
McCoy's 5A + 10% FBS |
ZF4 |
Zebrafish embryonic fibroblast |
DMEM:F12 + 10% FBS, 28°C |