Table 1.
Cell lines
| Line | Description | Growth medium |
|---|---|---|
| 247 | Primary human ovarian cancer cells isolated from ascites | RPMI-1640 + 10% FBS |
| 3T3 | Murine embryonic fibroblasts | DMEM + 10% FBS |
| 448 | Primary human ovarian cancer cells isolated from ascites | RPMI 1640 + 10% FBS |
| 4T1 | Murine breast carcinoma | DMEM + 10% FBS |
| 5637 | Human bladder carcinoma | RPMI 1640 + 10% FBS |
| 5Y | Human neuroblastoma | MEM:F12 + 2 mM l-glutamine + NEAA + 10% FBS |
| 67NR, 168FARN, 4T07, 66cl4 | Murine breast cancer progression series. 67NR forms primary tumors; 168FARN forms micrometastases in lymph nodes and 4T07 micrometastases to blood, lymph nodes and lungs. 66cl4 and 4T1 are fully metastatic and forms visible lung nodules (14, 15) | DMEM + 10% FBS |
| BE2 | Human neuroblastoma | MEM:F12 + 2 mM l-glutamine + NEAA + 10% FBS |
| Cos-7 | African green monkey kidney fibroblast-like | DMEM + 10% FBS |
| DOV13 | Human ovarian cancer | RPMI-1640 + 10% FBS |
| HaCaT | Human nontransformed keratinocyte | DMEM + 10% FBS |
| Hek293 | Human nontransformed embryonic kidney | DMEM + 10% FBS |
| Hep3B | Human hepatocellular carcinoma | MEM + 10% FBS |
| HepG2 | Human hepatocellular carcinoma | MEM + 10% FBS |
| HEY | Human ovarian cancer | RPMI-1640 + 10% FBS |
| HMEC | Nontransformed human mammary epithelial | DMEM + 10 μg/ml insulin + 10% FBS |
| HMEC-1 | Human dermal microvascular endothelial | MCDB-131 + 10% FBS + 1 μg/ml hydrocortisone + 10 ng/ml EGF + 2 mM l-glutamine |
| HMEpC | Primary human mammary epithelial cells (purchased from Cell Applications, Inc.) | Mammary epithelial growth medium (Cell Applications, Inc.) |
| HPrEpC | Primary human prostate epithelial cells (purchased from Cell Applications, Inc.) | Epi growth medium (Cell Applications, Inc.) |
| HT-29 | Human colorectal adenocarcinoma | McCoy's 5A + 10% FBS |
| IGROV | Human ovarian cancer | RPMI-1640 + 10% FBS |
| L6 | Rat myoblast | DMEM + 10% FBS |
| Mammary epithelial cells (TβRII WT and KO) | Nontransformed murine mammary epithelial | DMEM:F12 + 2.5 mM l-glutamine + 10% adult bovine serum (ABS) |
| Mammary fibroblast cells (TβRII WT and KO) | Nontransformed murine mammary fibroblast | DMEM:F12 + 2.5 mM l-glutamine + 10% ABS |
| MCF10A | Nontransformed human mammary epithelial | F12:DMEM + 10 μg/ml insulin + 0.5 μg/ml hydrocortisol + 20 ng/ml EGF + 100 ng/ml cholera toxin + 5% horse serum |
| MCF10A T1k, Ca1h, Ca1a | Human breast cancer progression series. MCF10A is nontumorigenic, T1k forms ducts that can develop hyperplastic lesions; Ca1h forms low-grade, well-differentiated carcinomas; and Ca1a forms poorly differentiated, metastatic carcinomas (16). | DMEM:F12 + 10 μg/ml insulin + 0.5 μg/ml hydrocortisol + 20 ng/ml EGF + 100 ng/ml cholera toxin + 5% horse serum |
| MCF7 | Human breast adenocarcinoma | MEM + 0.01 mg/ml insulin + 10% FBS |
| MDA-MB-231 | Human breast cancer | MEM + sodium pyruvate + nonessential amino acids (NEAA) + 10% FBS |
| MDCK | Nontransformed canine kidney epithelial | MEM + 10% FBS |
| MEEC | Murine embryonic endothelial | MCDB-131 + 15% FBS + 2 mM l-glutamine + 1 mM sodium pyruvate + 100 μg/ml heparin + 15 μg/ml endothelial cell growth supplement |
| Mv1Lu, DR, R1b | Nontransformed mink lung epithelial Mv1Lu is wild-type, DR lacks TβRII and ALK5, and R1b lacks ALK5 (17) | DMEM + 10% FBS |
| NMuMG | Murine nontransformed mammary epithelial | DMEM + 4.5g/l-glucose + 10 mg/ml insulin + 10% FBS |
| NOSE7 | Normal ovarian surface epithelial | RPMI-1640 + 10% FBS |
| Ovca 429 | Human ovarian cancer | RPMI + 10% FBS |
| Panc-1 | Human pancreatic cancer | DMEM + 10% FBS |
| PASMC | Murine pulmonary artery smooth muscle cells | RPMI-1640 + 10% FBS |
| PC3 | Human prostate adenocarcinoma | F12 + 10% FBS |
| RT4 | Human transitional cell papilloma | McCoy's 5A + 10% FBS |
| SHEP | Human neuroblastoma | DMEM + 2 mM l-glutamine + NEAA + 10% FBS |
| SJD.1 | Adult zebrafish caudal fin fibroblast | High glucose DMEM + 15% heat inactivated FBS, 28 °C |
| SKNAS | Human neuroblastoma | DMEM + 2 mM l-glutamine + NEAA + 10% FBS |
| SW480 | Human colorectal adenocarcinoma | DMEM + 10% FBS |
| SW620 | Human colorectal adenocarcinoma | DMEM + 10% FBS |
| T24 | Human transitional cell carcinoma | McCoy's 5A + 10% FBS |
| ZF4 | Zebrafish embryonic fibroblast | DMEM:F12 + 10% FBS, 28°C |