Table 1.
Probe | CFP-based fluorescent domain |
T391a | Citrine-based fluorescent domain |
FSK response, ΔF/F (%; N, n) |
|||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
K26 | F64 | S65 | Y66 | N146 | M153 | V163 | N164 | A206 | S65 | Y66 | V68 | Q69 | S72 | Y145 | H148 | T203 | H231 | BHK | Neurons | ||
AKAR2.1 | R | L | T | W | I | T | A | H | G | L | M | A | Y | I | NT | NT | |||||
GAkdY | R | L | T | I | T | A | H | G | L | M | A | W | V | Y | I | 32 ± 12; 8, 53 | 44 ± 10; 3, 20 | ||||
GAkmutdY | R | L | T | I | T | A | H | A | G | L | M | A | W | V | Y | I | NR; 3, 27 | NT | |||
GmutAkdYmut | R | L | G | G | I | T | A | H | G | G | L | M | A | W | V | Y | I | NF; 5, 0 | NT | ||
GAkdYmut | R | L | T | I | T | A | H | G | G | L | M | A | W | V | Y | I | 40 ± 16; 10, 59 | 74 ± 18; 7, 19 | |||
GAk | R | L | T | I | T | A | H | 26 ± 10; 8, 52 | 47 ± 9; 6, 15 | ||||||||||||
GA206KAk | R | L | T | I | T | A | H | K | 31 ± 11; 5, 27 | NT | |||||||||||
eGAk | L | T | A | NR; 5, 34 | NT | ||||||||||||||||
GI146NAk | R | L | T | T | A | H | NR; 5, 46 | NT | |||||||||||||
GR26KAk | L | T | I | T | A | H | 28 ± 12; 5, 24 | NT | |||||||||||||
GT153MAk | R | L | T | I | A | H | 28 ± 12; 7, 29 | NT | |||||||||||||
GH164NAk | R | L | T | I | T | A | 27 ± 9; 5, 25 | NT | |||||||||||||
CAk | R | L | T | W | I | T | A | H | −20 ± 6; 7, 51 | −16 ± 5; 7, 21 | |||||||||||
GmutAkdY | R | L | G | G | I | T | A | H | G | L | M | A | W | V | Y | I | −17 ± 8; 7, 50 | −19 ± 7; 8, 31 | |||
GmutAkY | R | L | G | G | I | T | A | H | G | L | M | A | Y | I | NR; 7, 63 | NT |
Mutations refer to the sequence of wild-type GFP (position 47; accession no. M62653). Numbering begins with the start methionine of each fluorescent domain except for the mutation in the PKA-sensing domain. Citrine-based fluorescent domain is absent where all columns are blank. ΔF/F values are means ± sd. N, total number of experiments n, total number of cells; NF, not fluorescent; NR, not responsive; NT, not tested.
PKA sensor domain.