Skip to main content
. 2014 Mar;28(3):1166–1180. doi: 10.1096/fj.13-240986

Figure 3.

Figure 3.

Quiescent cultures replicate vascular synthesis roles. A) SEM imaging of PC-deposited protein on glass demonstrates fibrous tracks, after substrate decellularization. B) LI-COR analysis of collagen and laminin content in PC-deposited protein on glass coverslips after decellularization. ***P < 0.001; unpaired 2-tailed t test. C, D) Collagen-specific (red; C) and laminin-specific (green; D) fluorescent stains, displaying the physiological heterogeneous protein deposition. Scale bars = 10 μm. E) IL-8 concentrations in the EC monolayer, PC monolayer, and EC/PC bilayer standardized to an equal volume of supernatant per cell. IL-8 was significantly higher in the EC monolayer than in the PC monolayer and the EC/PC bilayer. **P < 0.01, ***P < 0.001; 1-way ANOVA with the Tukey post hoc test.