Table 2.
Template and primers used for Siglec cloning
| Siglec | Template (BAC) | Forward primer | Reverse primer | Restriction digestion |
|---|---|---|---|---|
| Hsa Siglec-3 | CTD-3187F8 | HsaSig3-F1 | HsaSig3-R1 | NheI |
| Hsa Siglec-5 | CTC-470E3 | HsaSig5-F1 | HsaSig5-R1 | XbaI |
| Hsa Siglec-9 | CTD-3187F8 | HsaSig9-F1 | HsaSig9-R1 | XbaI |
| Ptr Siglec-3 | CH251-126O24 | HsaSig3-F1 | HsaSig3-R1 | NheI |
| Ptr Siglec-5 | CH251-426A12 | HsaSig5-F1 | HsaSig5-R1 | XbaI |
| Ptr Siglec-9 | CH251-126O24 | HsaSig9-F1 | HsaSig9-R1 | XbaI |
| Pcy Siglec-3 | RP41-94H15 | PcySig3-F1 | PcySig3-R1 | XbaI |
| Pcy Siglec-5 | RP41-126M5 | HsaSig5-F1 | HsaSig5-R1 | XbaI |
| Pcy Siglec-9 | RP41-94H15 | HsaSig9-F1 | HsaSig9-R1 | XbaI |
Has, human (for Homo sapiens); Ptr, chimpanzee (for Pan troglodytes); Pcy, baboon (for Papio cynocephalus anubis). See Table 3 for primer sequences.