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. 2014 Feb 19;9(2):e89326. doi: 10.1371/journal.pone.0089326

Figure 6. Confocal images showing the BrdUimmunostaining in lectin-positive BV2 microglia (A,C,E,G; red).

Figure 6

The number of BrdUpositive microglial cells (C,G: arrows) appears to be decreased at 24 h after Runx1t1 knockdown when compared to the cells transfected with scrambled siRNA. Nuclei are stained with DAPI. Scale bars: 50 µm. I. Quantitative analysis shows a significant decrease in number of BrdUimmunoreactive cells in activated microglia cultures transfected with Runx1t1siRNA for 24 h. Significant differences between groups (scrambled siRNA vs Runx1t1 siRNA, scrambled siRNA +LPS vs Runx1t1 siRNA+LPS) are indicated by *p<0.05, **p<0.01 (n = 3).