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. 2014 Feb 19;9(2):e89355. doi: 10.1371/journal.pone.0089355

Figure 3. Effect of iron reduction on oxidative stress in the kidneys of mice with UUO.

Figure 3

(A) Renal NADPH activity. Data are expressed as the mean ± SEM. *P<0.05. n = 4–6 per group. (B) Immunoblot analysis for p22phox and NOX4 protein expression. Upper panels: representative immunoblotting for p22phox and NOX4 expression. Lower panels: quantitative densitometry analysis of p22phox and NOX4 normalized to tubulin. Values are expressed as the mean ± SEM. *P<0.05, **P<0.01. n = 12 per group. (C) Representative immunohistochemical staining of p22phox expression.