Figure 3.

Cytospin preparations of human embryonic kidney293T cells transfected with different expression plasmids encoding the human G protein-coupled receptors RXFP1, RXFP2, or HE6 (as indicated), and then subjected to immunocytochemistry using the double-peroxidase anti-peroxidase technique and polyclonal antibodies raised against the peptide antigen L7–2 (amino acids 278–292 of the human RXFP1 sequence), coupled to KLH. In panels A and B antibodies derived from 2 independent rabbits immunized with the same L7–2 antigen were used. Note in these panels using transiently transfected cells that there are both labeled (dark stain) and unlabeled (pale cell outlines) cells. Panels C and D represent negative controls with cells transfected with plasmids for non-cognate receptors. See Ivell et al (20) for more details.