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A, FACS of dissociated ileum from LepRb-tomato reporter mice revealed these cells to be 0.25% to 0.5% of the total cell population within the ileum. B, Quantitative analysis of LepRb expression relative to GAPDH in unsorted but dissociated cells, tomato fluorescent “positive” cells and tomato fluorescent “negative” cells confirm that the tomato-positive cells are the predominant source of LepRb mRNA within the intestine. pos, positive; neg, negative.
