Figure 3.

A and B, Representative transmission electron micrographs of gastrocnemius muscles from mice fed an HFD (A) and mice receiving acipimox plus HFD (B) show normal ultrastructural myofibrillar architecture, with normal morphology of both SS and IMF mitochondria. C, As expected, nicotine plus HFD treatment shows IMF mitochondrial abnormalities, including IMCL accumulation. D, Acipimox treatment fully prevents nicotine plus HFD-induced IMCL accumulation and mitochondrial abnormalities. Scale bar, 1 μm. Data are representative of 4 mice in each group.