Figure 2.

The effect of intra-Arc rAAV-Kisspeptin-AS administration on oestrous cyclicity in adult female Wistar rats. Vaginal cytology was analyzed daily from day 4 to 100 after injection to determine oestrous cycle stage. Number of complete cycles (A), regular cycles (B), and average number of days per cycle (C) were calculated. D, Correlation between oestrous cycle length and ARC-kisspeptin expression in both the EGFP (○) and Kisspeptin-AS groups (■). Data are presented as mean ± SEM; *, P < .05; **, P < .01 vs rAAV-EGFP group, n = 12–14 per group.