Figure 3.

The effect of intra-Arc rAAV-Kisspeptin-AS administration on pulsatile LH release in OXV+E₂ female Wistar rats. Representative LH profiles illustrating the effect of rAAV-EGFP (A) or rAAV-EGFP and rAAV-Kisspeptin-AS (B) administration into the ARC on LH secretion in OVX+E₂ rats. ARC administration of rAAV-KP-AS resulted in a decrease in LH pulses frequency compared with rAAV-EGFP (C), with no effect on LH pulse amplitude (D). E, Correlation between LH pulse frequency and ARC-kisspeptin expression in both the EGFP (○) and Kisspeptin-AS groups (■); *, P < .05 vs rAAV-EGFP (n = 5–7).