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. 2014 Feb 19;9(2):e88936. doi: 10.1371/journal.pone.0088936

Table 1. Insertion sequence (IS)-content used in the species-specific qPCR assay.

IS481 IS1001 hIS1001 Ct used to determine positivitya Strain used for validation
B. pertussis + − − <25 ATCC strains n°12742
B. parapertussis − + − <25 CIP107610 strain, Pasteur Institute, Paris, France
B. holmesii + − + <25 CIP104394 strain, Pasteur Institute, Paris, Franceb
B. bronchiseptica −c −c − <25 QCMD strain Glasgow

qPCR: quantitative polymerase chain reaction; B: Bordetella; Ct: cycle threshold; IS: insertion sequence.

a

An amount of 1 ng of DNA purified from stored samples was subjected to qPCR.

Serial dilutions of purified DNA from control isolates were used to evaluate the sensitivity of the different PCR reactions, which were approximately 7–8 bacteria/reaction.

b

Kindly provided by Pr. N. Guiso.

c

Human and animal isolates of B. bronchiseptica can sometimes contain few copies of IS481 and/or IS1001 [22], [29].