Table 2. Sequence and characteristics of oligonucleotides used in the qPCR assay.
| Target | Primers-Probes names | Sequence 5′ to 3′ | Stock concentration [µM] | Concentration [µM] | Dye Reporter |
| IS481-109F | CCGGATGAACACCCATAAGC | 100 | 0.2 | ||
| B. pertussis | IS481-179R | CGATCAATTGCTGGACCATTT | 100 | 0.2 | |
| IS481-130T | TGCCCGATTGACCTTCCTACGTCGA | 100 | 0.1 | FAM | |
| IS1001-202F | CGCATCAGATAAGCGGTGAG | 100 | 0.2 | ||
| B. holmesii | IS1001-272R | CCGTGCCAATCGGTAAAGTT | 100 | 0.2 | |
| IS1001-holm-223T | AAGGGCTGGTTGGCCTGGAGCA | 100 | 0.1 | Texas Red | |
| IS1001parapertu-1001F | GTCCTGCGTGACGAACTCAA | 100 | 0.2 | ||
| B. parapertussis | IS1001parapertu-1071R | TGGTTCCAGGCTTGTCTTGC | 100 | 0.2 | |
| IS1001parapertu-1022T | CGGCTCTGGTTCTACCAAAGACCTGCC | 100 | 0.1 | Atto 700 | |
| Toxins-187F | AACGACAATGTGCTCGACCA | 100 | 0.2 | ||
| Toxins | Toxins-257R | GAGACGAAAGCGCTGTTGCT | 100 | 0.2 | |
| Toxins-208T | CTGACCGGACGTTCCTGCCAGG | 100 | 0.1 | FAM |
qPCR: quantitative polymerase chain reaction.