Table 4. Robustness of the proposed HPTLC method (n=6).
Conc. (ng/spot) | Mobile phase composition (Ethyl acetate: methanol: water) |
Results |
||||
Original | Used | Area±SD | % RSD | Rf | ||
15:3.2.1 | +0.1 | 7310±75 | 1.02 | 0.41 | ||
400 | 15:3:2 | 15:3:2 | 0.0 | 7290±59 | 0.81 | 0.40 |
15:3.1.9 | -0.1 | 7284±51 | 0.69 | 0.39 |
Conc.: Concentration.