Figure 1.

H. volcanii growth on eDNA as a C, N and/or P source. (A) Increase in OD during two growth phases after starvation (as in growth curve below) with eDNA supplementation in Hv-min medium containing all possible combinations of C, N and P. (B) After a 6 day starvation period (shown in smaller gray box) cultures were supplemented with C and N (squares) or a negative control starvation solution (circles) at T = 0 days (point 1) with OD measurements taken every hour. After one round of growth to stationary phase, KH₂PO₄ (purple filled squares) was added to CN cultures as a P source at point 2 and induced a second growth phase. Error bars are SD of replicate cultures.