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. 2014 Feb 20;5:57. doi: 10.3389/fmicb.2014.00057

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Copyright © 2014 Chimileski, Dolas, Naor, Gophna and Papke.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assay for secreted unbound nuclease activity in H. volcanii. Conditioned media was harvested from H. volcanii and S. aureus cultures, supplemented with plasmid DNA, and incubated at 37°C. Gel electrophoresis of DNA samples recovered after 12 h of incubation with CM is shown. Lane numbers are (1) 2-log DNA ladder (NEB N3200L), (2) DNA incubated with unconditioned LB medium, (3) DNA incubated with DNase I, (4) S. aureus CM, (5) S. aureus CM incubated with DNA, (6) H. volcanii CM, and (7) H. volcanii CM incubated with DNA.