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. 2014 Feb 20;5:57. doi: 10.3389/fmicb.2014.00057

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Copyright © 2014 Chimileski, Dolas, Naor, Gophna and Papke.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hvo_1477 is required for growth on DNA. (A) Deletion of chromosomal gene Hvo_1477 in H. volcanii strain H26. PCR amplification of H26 and Δ Hvo_1477 template DNA using the forward primer for the upstream Hvo_1477 flanking region, and the reverse primer of the downstream flanking region (Hvo_1477FR1_F and Hvo_1477FR2_R, Table 3). (B) Growth with eDNA in CN media for H26 (parental strain, black filled squares), Δ Hvo_1477 (red filled triangles) and Δ Hvo_1477 with pKD409_1477c complementation plasmid (Δ Hvo_1477c, purple circles). OD was measured every 3 h within a shaking and incubated 96-well plate reader. (C) Increase in optical density 96 h after supplementation with KH₂PO₄, DNA solvent (continued starvation), or eDNA. Error bars represent standard deviation of triplicate cultures.