Table 1.
Strains and plasmids.
| Strain or plasmid | Relevant properties | References or sources |
|---|---|---|
| PLASMIDS | ||
| pTA131 | Integrative vector based on pBluescript II; AmpR with BamHI-XbaI fragment from pGB70 containing pfdx-pyrE2 | Allers et al., 2004 |
| pTA409 | Shuttle vector based on pBluescript II, with pyrE2 and hdrB markers and ori-pHV1/4 replication origin | Hölzle et al., 2008 |
| pKD131_Δ1477 | pTA131 with HindIII-NotI fragment containing Hvo_1477 flanking regions for pop-in pop-out gene deletion | This study |
| pKD409_1477c | pTA409 with BamHI-EcoRI fragment containing Hvo_1477 and native upstream promoter | This study |
| STRAINS | ||
| E. coli | ||
| DH5α | Used for subcloning and as source of methylated E. coli DNA | Invitrogen, 18263012 |
| K12 | dam−/dcm− strain; used for source of unmethylated DNA | New England Biolabs, C2925I |
| H. volcanii | ||
| DS2 | Wild-type | Mullakhanbhai and Larsen, 1975 |
| H26 | Δ pyrE2 | Allers et al., 2004 |
| Δ Hvo_1477 | Hvo_1477 deletion in H26 background | This study |
| Δ Hvo_1477c | Δ Hvo_1477 with pKD409_1477c | This study |