Table 3.
Oligonucleotide primers used.
| Primer | Sequences (5′ −3′)a | Properties |
|---|---|---|
| Hvo_1477FR1_F | TTTAAGCTTCGTGCGCCCGATTTCCTTCT | Hvo_1477 deletion, upstream region external, HindIII site |
| Hvo_1477FR1_R | TTTGAATTCTCGATTCACCGTTAGTCAGGG | Hvo_1477 deletion, upstream region internal, EcoRI site |
| Hvo_1477FR2_F | AAAGAATTCCGACGAAGTGCTCGCGTACA | Hvo_1477 deletion, upstream downstream region internal, EcoRI site |
| Hvo_1477FR2_R | TTTGCGGCCGCCGAGATGCGCGGCGAGGT | Hvo_1477 deletion, downstream region external, NotI site |
| Hvo_1477P_F | AAAGGATCCGTTCCATTAAAAGGTTTCTGGT | Forward complementation, with native promoter, BamHI site |
| Hvo_1477_R | AAAGAATTCCAGTGTCTCCCCGAACAGCGG | Complementation, reverse, EcoRI site |
a
Restriction endonuclease sites are underlined.