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Mutation analysis by Sanger sequencing. The genes TERT (A), GNA11 (B) and EIF1AX (C) were found to be mutated as indicated on the left of each diagram by Sanger sequencing using the primers enlisted in Supplementary Table 2. The asterisk indicates the mutated position. No mutations have been detected in GNAQ, BAP1 and SF3B1 (see text).
