Figure 6.
JMJD2B silencing in vivo significantly suppressed CRC xenografts growth and led to γH2AX accumulation. (A) Effect of JMJD2B depletion on the tumour volumes of xenografts in nude mice. Abbreviations: si-NC, the negative control siRNA; si-JMJD2B, the siRNA-targeted JMJD2B. (B) Loss of JMJD2B reduces tumour growth rate. A total of 1.0 × 107 HCT116 cells were injected into the right flank of BALB/c nude mice. After the tumours grew to 5 mm in diameter, a negative control or JMJD2B siRNA mixed with in vivo-jetPEI transfection reagent, respectively, were injected intratumourally every other day for 11 days. Tumour volumes were measured at the indicated intervals. The tumour volume data are presented as means±s.d. *P<0.05, **P<0.01 vs si-NC. (C) JMJD2B knockdown led to γH2AX accumulation. Representative immunohistochemistry of JMJD2B and γH2AX in negative control (upper) and JMJD2B silencing (lower) tumour tissues. Abbreviation: HE=haematoxylin and eosin staining.