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. 2013 Oct 23;3(1):42–54. doi: 10.1016/j.molmet.2013.10.003

Figure 5.

Figure 5

Up-regulation of CG9510 increases cold tolerance, decreases stored triglyceride levels. (A) Survival following cold stress was counted for flies with up-regulated CG9510 (;da-GAL4/UAS-CG9510) and control flies (w1118;+/UAS-CG9510) after one week on RD or HFD. (B) Triglyceride (TG) levels (per mg of bodyweight) were determined in whole-body homogenate of flies with up-regulated CG9510 expression compared to control. Adult female flies (d3-5) from the GAL4-control cross (w1118;+/da-GAL4) or UAS-control cross (w1118;+/UAS-CG9510) were placed on RD and progeny from the experimental cross (;UAS-CG9510/da-GAL4) were placed either RD or HFD for one week prior to the assay. (C and D) Transgenic flies with rescued CG9510 expression (;P/P;UAS-CG9510/da-GAL4, where “P”=CG9510-PBac) and their controls (;P/P;+/UAS-CG9510 and;P/P;+/da-GAL4) were assayed for cold tolerance (C) and TG levels in whole-body homogenate (D) after one week on RD. Flies were tested in sets of 10 females per vial for cold tolerance, 5 females for TG levels: (A) n=6 sets (60 flies per group), (B) n=15–20 sets (75–100 flies per group), (C) n=4 sets (40 flies per group), (D) n=9–16 sets (45–80 flies per group). Error bars indicate standard error. Significance between values for the experimental group and control was determined with a t-test, *=p<0.05 **=p<0.01 ***=p<0.01 ****=p<0.0001.