Figure 2.

Altered gene expression caused by skeletal muscle-specific Bmal1 knockout. (A) Circadian expression profiles of core clock and clock-associated genes in TA muscles from control and mKO mice. Transcript levels were determined by microarray analysis and plotted as mean absolute expression levels (mean±SEM; n=3/group/timepoint). (B) Phase map of differentially expressed genes in control and mKO skeletal muscles (TA and SOL) identified by maSigPro (see Section 2). Muscles were collected at 4-h intervals throughout the light (ZT0-ZT8, white box) and dark phase (ZT12–ZT20, black box) and hybridized to Affymetrix arrays as described in the methods (n=3/group/timepoint).