Figure 3. Characterization of simulated cloud coverage and flow rate.

(a), temperature variation for denaturation, extension and annealing due to blocking of the light. Simulated clouding times ranged from 0 s (darkest curve) to 4 min (lightest curve) for a total test time of 27 min. The percentages represent the percent of time that each test spent in non-ideal PCR conditions, which for these tests was defined as when the denaturation temperature dropped below the dashed line. (b), gel electrophoresis and (c), corresponding band measurements show diminishing intensities as a function of the duration of simulated cloud coverage.