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. 2013 Oct 23;3(1):19–28. doi: 10.1016/j.molmet.2013.10.002

Figure 2.

Figure 2

Central FGF19 delivery improves whole body energy balance in HFD-fed and ob/ob mice. (A) Representative sections showing pERK1/2 (red) and co-localization between Npy-GFP positive neurons (green) and p-ERK1/2 positive cells (red) in ARC of HFD-fed Npy-GFP males 90 min after icv injection of aCSF and FGF19 (n=3). (B) Body weight (BW) change of HFD-fed males after 4 daily icv injections of aCSF or FGF19 (n=9 per group). (C) Food intake per mouse was measured 8 and 24 h post-icv injection of aCSF or 2 μg of FGF19 in HFD-fed males (n=5 per group). (D) GTT, with intraperitoneal (ip) injection of 1 g of glucose/kg of body weight (BW), was performed on HFD-fed males, after 6 h of fast (n=4–5 per group). The last icv injection of aCSF or FGF19 was performed 3 h before the GTT assay. (E) Serum insulin concentrations were assessed before and 15 min into the GTT in HFD-fed males treated with 4 daily icv injections of aCSF or FGF19 (n=5 per group). (F) ITT was performed with 0.5 U of insulin/kg of BW in HFD-fed males treated with 4 daily icv injections of aCSF or FGF19, after 6 h of fast (n=4 per group).The last icv injection of aCSF or FGF19 was performed 3 h before the ITT assay. (G) GTT, with ip injection of 0.5 g of glucose/kg of BW, was performed on ob/ob males fasted for 18 h and 3 h after the fourth icv injection (n=6 per group). (H) Insulinemia was assessed before and 15 min into the GTT (n=6 per group). (I) ITT was performed with 3 U of insulin/kg of BW in ob/ob males, after 6 h of fast and 3 h after the fourth icv injection (n=4 per group). *P<0.05, **P<0.005, and ***P<0.0005. Scale bars: 100 μm.