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Tissue-specific expression of channelrhodopsin in DA neurons of ob/ob mice and cranial fiber optic implant. (a–c) AAV-DIO–ChR2-mCherry injection into the VTA of ob/ob;Dat-cre mice led to ChR2-mCherry expression in neurons colocalizing with tyrosine hydroxylase (TH), a marker for DA neurons (statistics imbedded in text, scale bars represent 100 µm). (d and e) Optical fibers were implanted above the VTA (red inset box in (d)) of ob/ob;Dat-cre mice.
