Table 2.

Oligonucleotide primer pairs that anneal within the pheromone gland fatty-acyl reductase, pgfar, gene of Ostrinia nubilalis to prime the PCR amplification of regions that contain pheromone race-specific single nucleotide polymorphisms (SNPs)

Name	Oligonucleotide primer	SNP	RE	Allele specific
pgFAR-tf	5′-TTC GAT TCG GGA ACC CAT A-3′	T857	TaqI(+)	pgfar-z
pgFAR-tr	5′-AGG TTC GCA ACG TGG TCT AC-3′	G857	TaqI(–)	pgfar-e
pgFAR-mnf	5′-G GGC AAC AAA GGA GTC AAG GT-3′	T995	NdeII(+)	pgfar-e
pgFAR-mnr	5′-CC AAA ATA TTT CCT GTA TTT TAW GCA-3′	G995	NdeII(–)	pgfar-z
pgFAR-mnf	5′-G GGC AAC AAA GGA GTC AAG GT-3′	T1005	MseI(+)	pgfar-e
pgFAR-mnr	5′-CC AAA ATA TTT CCT GTA TTT TAW GCA-3′	G1005	MseI(–)	pgfar-z

The SNPs are indicated as the nucleotide position within the pgfar cDNA and alternate nucleotides present at the locus (nucleotide ^position), and the restriction enzyme (RE) used to detect each allele are indicated. G/T⁹⁹⁵ and G/T¹⁰⁰⁵ are detected by PCR-RFLP of the same PCR-amplified fragment.

TaqI(–) 150 bp, TaqI(+) 118 + 32 bp; NdeII(–) 145 bp, NdeII(+) 83 + 62 bp; MseI(–) 145 bp, MseI(+) 93 + 52 bp.