Table 1.
Normalized cDNA library | Nonnormalized cDNA library | |
---|---|---|
Total reads | 252,186,716 | 79,030,219 |
Mapped reads | 1,787,162 | 3,159,679 |
Total SNPs called | 1,014,088 | 755,147 |
Heterozygous SNP | 50,124 | 30,682 |
SNPs (coverage ≥10) | 611,971 | 415,411 |
Heterozygous (coverage ≥10) | 45,645 | 27,143 |
SNPs (coverage ≥100) | 181,683 | 138,058 |
Heterozygous (coverage ≥100) | 17,765 | 9812 |
Read mapping was performed using the sturgeon EST database as a reference. SNP calling was done with default SNP caller settings (using SAMtools) and with minimum coverage depths of 10 and 100.