Fig. 4.

Calmodulin is required for the colocalization of MyoIIA and F-actin. (A) Mature 3T3-L1 adipocytes grown on coverslips were serum starved for 4 h and then either left untreated (basal), treated with 100 nM insulin for 15 min, or treated with W13 (70 μM) for 15 min and then stimulated with 100 nM insulin for 15 min. Confocal microscopy was used to visualize the localization of MyoIIA and F-actin. White arrows indicate either cytoplasmic or cell cortex localization. (B) Distribution of GLUT4, MyoIIA, and F-actin within the cell and at the cortex under basal, insulin or W13 conditions was quantified as described in the Materials and methods. Images are representative of 3 independent experiments.