Figure 1. Effects of the MIS-MΦs on T cell production of various cytokines.

(a), (b) Anti-CD3 Ab/Anti-CD28 Ab-stimulated T cells (TCR-stimulated T cells) were cultivated with or without a monolayer culture of MIS-MΦs for 72 hr and measured for proliferative response (a) and production of cytokines in culture fluids by ELISA (b). (c) TCR-stimulated T cells were cultured with or without a monolayer culture of MIS-MΦs for up to 7 days. At intervals, concentrations of IL-17 in culture fluids were measured. (d), (e) Cultured T cells harvested at the same time points were stained with PI (10 μg/ml) after RNase A (200 μg/ml) treatment, subjected to cytofluorometry (d). The ratio of apoptotic T cells was estimated based on the ratio of the sub-G₁ cell population (e). Data are representative of multiple experiments; error bars, s.e.m.; **p < 0.01, *p < 0.05 (Bonferroni's multiple t-test).