Figure 12.

Pirfenidone regulates chemokine (CC motif) receptor-2 (CCR2) in fibrocytes. Isolated fibrocytes were cultured in the presence or absence of pirfenidone (100 μg/mL) for 48 h. Then, total RNA was extracted and converted to complementary DNA. Quantitative real-time polymerase chain reaction was performed to evaluate CCR2 messenger RNA (mRNA) expression. CCR2 mRNA was significantly attenuated by pirfenidone (*P = 0.0329). Data are means ± standard error from 4 separate experiments, each assayed in triplicate. PFD, pirfenidone.