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. 2014 Feb 8;15(1):16. doi: 10.1186/1465-9921-15-16

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Copyright © 2014 Inomata et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Immunohistochemical analyses. Immunohistochemical analyses for CD45 (green; A, D, and G) and collagen-I (Col-I; red; B, E, and H) staining were performed with lung sections on day 14 of BLM treatment with or without pirfenidone. Merge (yellow; C, F, and I) is co-staining for CD45 and Col-I, indicating bone marrow-derived fibrocytes. Fibrocytes are indicated with arrows. The increased numbers of fibrocytes expressing CD45 and Col-I observed in BLM-treated mice were attenuated by pirfenidone treatment. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole. Original magnification, ×40. PFD, pirfenidone.