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. 2013 Oct 17;8(3):685–698. doi: 10.1038/ismej.2013.171

Figure 1.

Figure 1

Time series of quarterly sampling at Marsh Landing, Sapelo Island, GA, USA. Data are from 4–8 replicate samples of surface water collected over a 48 h period and thus reflect variability associated with local patchiness. (a) qPCR measurements of the relative abundance of Thaumarchaeota rrs (○) and Archaeal amoA genes (□); (b) qPCR measurements of the abundance of Nitrospina rrs (○) and Bacterial amoA (□) genes; (c) contribution of Thaumarchaeota transcripts to metatranscriptomes (○); (d) contribution of transcripts from nitrite- (NOB; ○) or ammonia (AOB; □)-oxidizing Bacteria to metatranscriptomes. Relative abundances in a and b are calculated as in Kalanetra et al. (2009), hit counts in c and d are normalized as percentages of RefSeq hits (Bacteria plus Archaea, all prokaryotes) in each metatranscriptome.